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Proteomic Analysis of Bovine Nucleolus - Proteomic Analysis of Bovine Nucleolus

Amrutlal ; K. ; Patel ; Doug ; Olson ; Suresh ; K. ; Tikoo

基因组蛋白质组与生物信息学报:英文版 - Genomics Proteomics & Bioinformatics, 2010, Vol.8(3), pp.145-158 [Rivista Peer Reviewed]

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  • Titolo:
    Proteomic Analysis of Bovine Nucleolus - Proteomic Analysis of Bovine Nucleolus
  • Autore: Amrutlal ; K. ; Patel ; Doug ; Olson ; Suresh ; K. ; Tikoo
  • Descrizione: Nucleolus is the most prominent subnuclear structure, which performs a wide variety of functions in the eukaryotic cellular processes. In order to understand the structural and functional role of the nucleoli in bovine cells, we analyzed the proteomic composition of the bovine nucleoli. The nucleoli were isolated from Madin Darby bovine kidney cells and subjected to proteomic analysis by LC-MS/MS after fractionation by SDS-PAGE and strong cation exchange chromatography. Analysis of the data using the Mascot database search and the GPM database search identified 311 proteins in the bovine nucleoli, which contained 22 proteins previously not identified in the proteomic analysis of human nucleoli. Analysis of the identified proteins using the GoMiner software suggested that the bovine nucleoli contained proteins involved in ribosomal biogenesis, cell cycle control, transcriptional, translational and post-translational regulation, transport, and structural organization.
  • Fa parte di: 基因组蛋白质组与生物信息学报:英文版 - Genomics Proteomics & Bioinformatics, 2010, Vol.8(3), pp.145-158
  • Soggetti: 蛋白质组分 ; 细胞核 ; LC ; MS/MS ; ; 核仁结构 ; 数据库检索 ; 细胞周期调控 ; 色谱分离 ; Bovine, Nucleolus, Proteomics, 1d SDS-Page, Scx, LC-MS/MS
  • Titoli correlati: 作者单位: Vaeeine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada National Research Council, Plant Biotechnology Institute, University of Saskatchewan, Saskatoon, Canada School of Public Health, University of Saskatchewan, Saskatoon, Canada.
  • Identificativo: ISSN: 1672-0229

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