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Chapter 2 - Hepatic Stellate Cell Culture Models

Rombouts, Krista

Stellate Cells in Health and Disease, Chapter 2, pp.15-27

Elsevier Inc. ; 2015

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  • Titolo:
    Chapter 2 - Hepatic Stellate Cell Culture Models
  • Autore: Rombouts, Krista
  • Editore: Elsevier Inc.
  • Anno: 2015
  • Descrizione: Hepatic stellate cells (HSCs) are perisinusoidal resident vitamin A-storing cells and are considered to be the most relevant profibrogenic cells operating in acute and chronic liver diseases. During liver injury, HSCs undergo phenotypic transformation from non-proliferating and non-contractile “quiescent” cells into “activated” promitogenic, profibrogenic, and proinflammatory myofibroblasts-like cells. Multiple etiologies of liver injury can lead to fibrosis, resulting from an imbalance between the production and degradation extracellular matrix (ECM). Thus, activated HSCs orchestrate an impressive response to different types of liver injury, leading to net deposition of ECM into the interstitium as a wound-healing reaction. In the last four decades several new and exciting in vitro models have been developed to mimic the normal microenvironment of HSCs in order to understand crucial molecular mechanisms of their activation that would be necessary to develop new clinical therapies. This chapter recapitulates the history of HSC isolation and provides an overview of various cell culture models. It also compares the differences between two-dimensional and three-dimensional in vitro models to investigate the characteristics of cultured quiescent and activated HSCs.
  • Fa parte di: Stellate Cells in Health and Disease, Chapter 2, pp.15-27
  • Soggetti: Hepatic stellate cells (HSCs) ; cell culture ; co-culture ; two-dimensional culture (2D) ; three-dimensional culture (3D) ; extracellular matrix (ECM) ; precision-cut liver slices (PCLS) ; bioscaffold ; Matrigel
  • Lingua: Inglese
  • Identificativo: ISBN: 978-0-12-800134-9 ; ISBN: 978-0-12-800544-6 ; DOI: 10.1016/B978-0-12-800134-9.00002-6
  • Fonte: ScienceDirect (Elsevier B.V.)

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