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Automated flow cytometry for monitoring CHO cell cultures

Kuystermans, Darrin ; Mohd, Avesh ; Al-Rubeai, Mohamed

Methods, March 2012, Vol.56(3), pp.358-365 [Rivista Peer Reviewed]

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  • Titolo:
    Automated flow cytometry for monitoring CHO cell cultures
  • Autore: Kuystermans, Darrin ; Mohd, Avesh ; Al-Rubeai, Mohamed
  • Descrizione: Flow cytometry has been used to accurately monitor cell events that indicate the spatio-temporal state of a bioreactor culture. The introduction of process analytical technology (PAT) has led to process improvements using real-time or semi real-time monitoring systems. Integration of flow cytometry into an automated scheme for improved process monitoring can benefit PAT in bioreactor-based biopharmaceutical productions by establishing optimum process conditions and better quality protocols. Herein, we provide detailed protocols for establishing an automated flow cytometry system that can be used to investigate and monitor cell growth, viability, cell size, and cell cycle data. A method is described for the use of such a system primarily focused on CHO cell culture, although it is foreseen the information gathered from automated flow cytometry can be applied to a variety of cell lines to address both PAT requirements and gain further understanding of complex biological systems.
  • Fa parte di: Methods, March 2012, Vol.56(3), pp.358-365
  • Soggetti: Flow Cytometry ; Automation ; Cell Cycle ; Cho Cells ; Process Analytical Technology ; Pat ; Monitoring ; Flowcytoprep ; Chemistry ; Anatomy & Physiology
  • Lingua: Inglese
  • Identificativo: ISSN: 1046-2023 ; E-ISSN: 1095-9130 ; DOI: 10.1016/j.ymeth.2012.03.001

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