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Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells

Tey, Beng Ti ; Al-Rubeai, Mohamed

Journal of Bioscience and Bioengineering, 2005, Vol.100(3), pp.303-310 [Rivista Peer Reviewed]

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  • Titolo:
    Effect of Bcl-2 overexpression on cell cycle and antibody productivity in chemostat cultures of myeloma NS0 cells
  • Autore: Tey, Beng Ti ; Al-Rubeai, Mohamed
  • Descrizione: Chemostat cultures of NS0 cell lines were carried out at dilution rates ranging from 0.8 d to 0.2 d . Compared with the control, the viable cell density of the Bcl-2 cell line was approximately 10% higher at 0.8 d and increased to 55% when the dilution rate was reduced to 0.2 d . As the dilution rate was reduced, the viability of the two cultures diverged reaching a difference of 43% at 0.2 d . The specific growth rate of the control cells was the same as the dilution rate down to a value of 0.6 d . By contrast, the specific growth rate of Bcl-2 cells was parallel to the dilution rate down to a value as low as 0.3 d . For both NS0 cell lines, the G cell population decreased, while the S and G /M cell populations increased as the dilution rate was reduced. The antibody titer of the control cells increased from 7 to 21 μg·ml as the dilution rate was reduced from 0.8 to 0.2 d . With an initial increase from 2 to 15 μg·ml as the dilution rate was reduced from 0.8 to 0.4 d , the antibody titer of the Bcl-2 cells remained constant as the dilution rate was further reduced to 0.2 d . A good correlation between specific antibody production rate and the percentage of G /M cells was observed.
  • Fa parte di: Journal of Bioscience and Bioengineering, 2005, Vol.100(3), pp.303-310
  • Soggetti: Apoptosis ; Bcl-2 ; Ns0 Myeloma Cells ; Bioreactor ; Chemostat Culture ; Biology
  • Lingua: Inglese
  • Identificativo: ISSN: 1389-1723 ; E-ISSN: 1347-4421 ; DOI: 10.1263/jbb.100.303

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