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Mathematical approach for the optimal expansion of erythroid progenitors in monolayer culture

Lahoti, Varun ; Murphy, William ; Al - Rubeai, Mohamed

Journal of Biotechnology, Oct 31, 2012, Vol.161(3), p.308(12) [Rivista Peer Reviewed]

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  • Titolo:
    Mathematical approach for the optimal expansion of erythroid progenitors in monolayer culture
  • Autore: Lahoti, Varun ; Murphy, William ; Al - Rubeai, Mohamed
  • Descrizione: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.jbiotec.2012.06.003 Byline: Varun Lahoti, William Murphy, Mohamed Al-Rubeai Keywords: Gompertz; Expansion CD34.sup.+; Erythroid; Monolayer culture; Mathematical Abstract: a* We examine culture conditions for inoculation and passaging of erythroid progenitors. a* We use mathematical expressions to define the growth curves of peripheral blood derived erythroid progenitors over the course of their expansion process. a* Significantly different growth curves are obtained at each passage confirming the altered growth kinetics of erythroid progenitors during sequential passaging in expansion process. a* Cost analysis suggest a 60h passage length at every passage, resulting in a 43% process-cost reduction. Author Affiliation: School of Chemical and Bioprocess Engineering, and Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland Article History: Received 27 January 2012; Revised 8 June 2012; Accepted 11 June 2012
  • Fa parte di: Journal of Biotechnology, Oct 31, 2012, Vol.161(3), p.308(12)
  • Soggetti: Monomolecular Films -- Analysis ; Universities And Colleges -- Analysis
  • Lingua: English
  • Identificativo: ISSN: 0168-1656
  • Fonte: Cengage Learning, Inc.

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