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General Outline of Techniques and Procedures

Yam, Tim Wing ; Arditti, Joseph

Micropropagation of Orchids, Micropropagation of Orchids, Chapter 2, p.73-168

Chichester, UK: John Wiley & Sons, Ltd ; 2017

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  • Titolo:
    General Outline of Techniques and Procedures
  • Autore: Yam, Tim Wing ; Arditti, Joseph
  • Editore: Chichester, UK: John Wiley & Sons, Ltd
  • Anno: 2017
  • Descrizione: methods for the in vitro culture of isolated plant cells, tissues, and organs or of seeds require appropriate equipment, certain skills and some knowledge. this chapter outlines these skills and the list of methods, media, and apparatus. culture media vary widely in the use and content of micro‐ or minor elements or nutrients. the reasons for this are utilization of existing formulations; imprecise and/or incomplete and/or anecdotal information regarding the requirements of orchids; and the presence of many of these elements as impurities in other media components. individual stock solutions should be prepared for each macroelement, vitamin, amino acid, or hormone. all microelements should be combined into one stock solution. culture media, tools, working space, and tissues must be sterilized, and work has to be carried out under aseptic conditions to ensure success. if these precautions are not taken, cultures will become contaminated and fail. a number of methods are used to ensure sterility.
  • Fa parte di: Micropropagation of Orchids, Micropropagation of Orchids, Chapter 2, p.73-168
  • Soggetti: Culture Media ; Culture Vessels ; Media Components ; Orchid Micropropagation ; Plant Material ; Plant Tissue Culture ; Sterilization ; Stock Solutions
  • Lingua: Inglese
  • Identificativo: ISBN: 9781119187059 ; E-ISBN: 9781119187080 ; DOI: 10.1002/9781119187080.ch2
  • Fonte: John Wiley & Sons, Inc.

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