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Transgenic Technology in Fish

Coastal and Estuarine Studies, Aquaculture: Fundamental and Applied Research, Chapter 17, p.275-286

Washington, D. C.: American Geophysical Union ; 2013

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  • Titolo:
    Transgenic Technology in Fish
  • Editore: Washington, D. C.: American Geophysical Union
  • Anno: 2013
  • Descrizione: biological experimentation in fish, as well as their genetic improvement for aquaculture could greatly benefit from in vivo gene transfer technologies, if convenient methods of gene introduction permit the integration of the exogenous sequences into their genome. diverse constraints have determined the choice of cytoplasmic injections to introduce the foreign genes in vivo. this system has a variable efficiency in different studies, but is now clearly validated for the integration. other methods, such as dna injections into the oocyte germinal vesicle followed by in vitro maturation, or such as the egg electroporation, have been also tested with more or less success. satisfactory expression levels of the transgenes have been obtained with reporter genes, and possibilities to manipulate their transcription level and the location of their expression are now permitted. however, the capacity to express any transgene in vivo remains a challenge, especially when heterologous gene constructs are utilized.
  • Fa parte di: Coastal and Estuarine Studies, Aquaculture: Fundamental and Applied Research, Chapter 17, p.275-286
  • Soggetti: Fishes—Physiology—Congresses ; Hatchery Fishes—Physiology—Congresses ; Fish‐Culture—Congresses ; Aquaculture—Congresses
  • Lingua: Inglese
  • Identificativo: ISBN: 9780875902579 ; E-ISBN: 9781118665107 ; DOI: 10.1029/CE043p0275
  • Fonte: John Wiley & Sons, Inc.

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